Role of the tryptophan residues in proton - coupled folate transporter ( PCFT - SLC 46 A 1 ) 1 function
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چکیده
Role of the tryptophan residues in proton-coupled folate transporter (PCFT-SLC46A1) 1 function 2 3 Mitra Najmi, Rongbao Zhao, Andras Fiser and I. David Goldman 4 5 Departments of Molecular Pharmacology, Medicine, Systems and Computational Biology, 6 Biochemistry, Albert Einstein College of Medicine, Bronx, New York 7 8 Running Head: Role of the tryptophan residues in PCFT function 9 10 Address for Correspondence: I. David Goldman, Departments of Molecular Pharmacology 11 and Medicine, Albert Einstein Cancer Center, Chanin 2, 1300 Morris Park Avenue, Bronx, NY 12 10461 (e-mail: [email protected]) 13 14 15 Abbreviations: DTT, dithiotreitol; HFM, hereditary folate malabsorption; MTSEA-biotin, N-biotinyl 16 aminoethylmethanethiosulfonate; MTX, methotrexate; PCFT, proton-coupled folate transporter; 17 SCAM, substituted-cysteine accessibility method; TMD, transmembrane domain. 18 19 20 21 22 Articles in PresS. Am J Physiol Cell Physiol (June 1, 2016). doi:10.1152/ajpcell.00084.2016
منابع مشابه
Role of the tryptophan residues in proton-coupled folate transporter (PCFT-SLC46A1) function.
The proton-coupled folate transporter (PCFT) mediates folate absorption across the brush-border membrane of the proximal small intestine and is required for folate transport across the choroid plexus into the cerebrospinal fluid. In this study, the functional role and accessibility of the seven PCFT Trp residues were assessed by the substituted-cysteine accessibility method. Six Trp residues at...
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The proton-coupled folate transporter (PCFT-SLC46A1) mediates intestinal folate absorption and folate transport across the choroid plexus, processes defective in hereditary folate malabsorption (HFM). This paper characterizes the functional defect, and the roles of two mutated PCFT residues, associated with HFM (G338R and A335D). The A335D-PCFT and other mutations at this residue result in an u...
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The proton-coupled folate transporter (PCFT, SLC46A1) mediates folate transport across the apical brush-border membrane of the proximal small intestine and the basolateral membrane of choroid plexus ependymal cells. Two loss-of-function mutations in PCFT, which are the basis for hereditary folate malabsorption, have been identified within the fourth transmembrane domain (TMD4) in subjects with ...
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The proton-coupled folate transporter (PCFT) mediates intestinal folate absorption and transport of folates across the choroid plexus. This study focuses on the role of Tyr residues in PCFT function. The substituted Cys-accessibility method identified four Tyr residues (Y291, Y362, Y315, and Y414) that are accessible to the extracellular compartment; three of these (Y291, Y362, and Y315) are lo...
متن کاملCloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types.
PURPOSE In a prior study the cellular uptake of folate was investigated in the retina. Recently, a new proton-coupled folate transporter (PCFT) in human intestine was reported. In the present study, the expression of this novel transporter in the retina was determined, the mouse orthologue was cloned from retinal tissue, and its transport function was characterized. METHODS RT-PCR and folate ...
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تاریخ انتشار 2016